An ambrotype of a father with his daughter on his lap. She looks like she has either very light brown or blond hair and very fair eyes.  Daddy seems to be very proud of her and she is definitely very mych alive. Her one hand has moved during the photo shoot so daddy is trying to keep her still so the picture will come out well. It is rare to find pictures of just daddy and his children. Hopefully his wife was still alive when this picture was taken. It measures 2.75" x 3.25" with the case hanging on by several threads.